Journal: The Journal of investigative dermatology
Article Title: Human beta-defensin-2 production in keratinocytes is regulated by interleukin-1, bacteria, and the state of differentiation.
doi: 10.1046/j.0022-202x.2001.01651.x
Figure Lengend Snippet: Figure 2. Antimicrobial activity of rhBD-2 against S. epidermidis, S. aureus, S. agalactiae, E. coli ML35p, P. aeruginosa, and C. albicans. The number of CFU was determined after a 1 h incubation with rhBD-2. Experiments made in low-salt or high-salt concentrations are indicated by black and open squares, respectively. Controls containing peptide solvent only (0.01% acetic acid) are also shown for incubation times of 0 and 1 h. If CFU concentrations changed signi®cantly during incubation with solvent only, arrows indicate whether the CFU increased or decreased compared to inoculum. Error bars indicate standard deviations of the mean of triplicate experiments.
Article Snippet: Microbial strains and antimicrobial assays The microbial strains used in this study, including the Gram-negative bacteria Escherichia coli ML35p (ampicillin-resistant strain) and Pseudomonas aeruginosa ATCC 9027, the Gram-positive Streptococcus agalactiae, Staphylococcus epidermidis, and S. aureus ATCC 6341, and the fungus Candida albicans 820, were kindly provided by Robert I. Lehrer (UCLA, School of Medicine, Los Angeles, CA).
Techniques: Activity Assay, Incubation, Solvent